[A study on inflammatory regulation of human gallbladder epithelial cells by PPAR-gamma ligand].

Abstract

OBJECTIVE:

To investigate the inflammatory regulation of human gallbladder epithelial cells (HGBEC) by peroxisome proliferator activated receptor gamma (PPAR-gamma) ligand ciglitazone.

METHODS:

HGBEC were cultured in medium containing human epidermal growth factor (hEGF). HIL-1beta were added into the ciglitazone groups and inflammatory control groups to make inflammatory model . IL-6 and TNF-alpha concentration in ciglitazone groups and all control groups were measured.

RESULTS:

HGBEC were cultured in medium successfully. The inflammatory model was made. The longest duration is 25 d. In inflammatory control groups, cells were edema with unclear cellular membrane and plasmid. In ciglitazone groups, the inflammatory edema of cells were less evident than that in inflammatory control groups, especially in 50 micromol/ml group. The IL-6 concentration in ciglitazone groups is lower than that in control group (P<0.01). The relation between the inhibitory effect and the concentration of ciglitazone is positive correlation.

CONCLUSION:

Ciglitazone that can inhibit the inflammation of HGBEC maybe an effective treatment for acute and chronic cholecystitis.

• Authors: Guang-dong Pan, Nan-sheng Cheng, Xian-ze Xiong, Jiang-wen Liu, Sheng-fu Li, Guo-fu Zahng, Lü-nan Yan
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 35 Issue 5 Pg. 658-61 (Sep 2004) ISSN: 1672-173X [Print] China
• PMID: 15460412 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Interleukin-1
• Interleukin-6
• Ligands
• PPAR gamma
• Thiazolidinediones
• Transcription Factors
• ciglitazone

Topics

• Anti-Inflammatory Agents (pharmacology)
• Cell Division (drug effects)
• Cells, Cultured
• Epithelial Cells (cytology)
• Gallbladder (cytology)
• Humans
• Interleukin-1 (pharmacology)
• Interleukin-6 (metabolism)
• Ligands
• PPAR gamma (antagonists & inhibitors)
• Thiazolidinediones (pharmacology)
• Transcription Factors (agonists, metabolism, physiology)