| Abstract | REASONS FOR PERFORMING STUDY: Hyaluronic acid (HA) is an endogenous glycosaminoglycan used in the treatment of joint diseases, but medication control is required by horseracing authorities. Therefore, a medication control policy needs to be established. OBJECTIVES: To establish physiological plasma HA concentrations in post race horses, determine the HA endogenous production rate and document the disposition of HA after i.v. and intra-articular hyaluronic acid administration at recommended therapeutic doses. METHODS: Hyaluronan concentrations in plasma were determined using an ELISA specific test; concentrations in synovial fluid were determined using a radiometric binding assay. RESULTS: The overall mean plasma HA concentration in 120 post competition horses was 89 ng/ml. In a group of 6 experimental horses, synovial fluid control concentration was 328+/-112 microg/ml. After i.v. sodium hyaluronate administration (37.8 mg in toto), the terminal half-life was very short (43+/-29 mins) and after a delay of 3 h, the plasma concentration returned to control values. The endogenous HA production rate was 33-164 mg in toto per day, i.e. 1-4 times the recommended i.v. daily dose. Twenty-four hours after intra-articular administration, HA concentration was not significantly different from control values (328+/-112 microg/ml). CONCLUSIONS AND POTENTIAL RELEVANCE: Due to the rapid disappearance of HA from plasma after i.v. administration and from the joint after intra-articular administration, long-term detection needs a more appropriate approach to be developed. |
| Authors | M A Popot, Y Bonnaire, J Guéchot, P L Toutain
(Affiliation: Laboratoire des Courses Hippiques, 15 Rue de Paradis, 91370 Verrières le Buisson, France.)
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| Journal | Equine veterinary journal
(Equine Vet J)
Vol. 36
Issue 6
Pg. 482-7
(Sep 2004)
ISSN: 0425-1644 England |
| PMID | 15460071
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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| Chemical References |
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| Topics |
- Analysis of Variance
- Animals
- Cross-Over Studies
- Enzyme-Linked Immunosorbent Assay
(veterinary)
- Female
- Horses
(blood, metabolism)
- Hyaluronic Acid
(biosynthesis, blood, pharmacokinetics)
- Injections, Intra-Articular
(veterinary)
- Injections, Intravenous
(veterinary)
- Male
- Physical Conditioning, Animal
(physiology)
- Radiometry
- Random Allocation
- Reference Values
- Synovial Fluid
(chemistry)
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