HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

In vitro anti-tumor activity of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone against six established human cancer cell lines.

Abstract
2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), isolated from the buds of Cleistocalyx operculatus, was investigated in its cytotoxicity and its influence on six human cancer cell lines. Among SMMC-7721, 8898, HeLa, SPC-A-1, 95-D and GBC-SD cell lines, SMMC-7721 cells was the most sensitive one in these tested cell lines, with IC50 equal to 32.3 +/- 1.13 microM, EC50 equal to 9.00 +/- 0.36 microM and the therapeutic index equal to 3.59. Staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the cells treated with 9 microM DMC for 48 h. Flow cytometric analysis was performed to determine hypodiploid cells. The results of flow cytometry assay indicated that the percentage of hypodiploid SMMC-7721 cells were 49.44 +/- 1.06% after 48 h treatment with 18.0 microM DMC. The treatment resulted in the appearance of a hypodiploid peak (A0 region), probably due to the presence of apoptosing cells and/or apoptotic bodies with DNA content less than 2n. To our knowledge, this is the first report on anti-tumor activity by DMC.
AuthorsChun-Lin Ye, Jian-Wen Liu, Dong-Zhi Wei, Yan-Hua Lu, Feng Qian
JournalPharmacological research (Pharmacol Res) Vol. 50 Issue 5 Pg. 505-10 (Nov 2004) ISSN: 1043-6618 [Print] Netherlands
PMID15458771 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 2',6'-dihydroxy-4'-methoxychalcone
  • Antineoplastic Agents, Phytogenic
  • Chalcones
  • Chalcone
Topics
  • Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
  • Cell Line, Tumor
  • Chalcone (analogs & derivatives, chemistry, pharmacology)
  • Chalcones
  • Dose-Response Relationship, Drug
  • Humans
  • Myrtaceae
  • Tumor Stem Cell Assay (statistics & numerical data)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: