Green tea constituent epigallocatechin-3-gallate selectively inhibits COX-2 without affecting COX-1 expression in human prostate carcinoma cells.

Abstract

Overexpression of cyclooxygenase (COX)-2 has been implicated in many pathologic conditions, including cancer. One practical inference of this finding is that sustained inhibition of COX-2 could serve as a promising target for prevention or therapy of cancer. Conventional nonsteroidal antiinflammatory drugs (NSAIDs) and recently developed COX-2-specific inhibitors have shown considerable promise in prevention of some forms of human cancer; however, its application is limited due to severe toxic side effects on normal cells. Therefore, there is a need to define novel, nontoxic dietary constituents with proven chemopreventive effects through other pathways that also possess COX-2 but not COX-1 inhibitory activity. Recent studies on green tea and its major polyphenolic constituent (-)epigallocatechin-3-gallate (EGCG) have established its remarkable cancer preventive and some cancer therapeutic effects. Here, we show that EGCG inhibits COX-2 without affecting COX-1 expression at both the mRNA and protein levels, in androgen-sensitive LNCaP and androgen-insensitive PC-3 human prostate carcinoma cells. Based on our study, it is tempting to suggest that a combination of EGCG with chemotherapeutic drugs could be an improved strategy for prevention and treatment of prostate cancer.

• Authors: Tajamul Hussain, Sanjay Gupta, Vaqar M Adhami, Hasan Mukhtar
• Journal: International journal of cancer (Int J Cancer) Vol. 113 Issue 4 Pg. 660-9 (Feb 10 2005) ISSN: 0020-7136 [Print] United States
• PMID: 15455372 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antineoplastic Agents
• Isoenzymes
• Membrane Proteins
• RNA, Messenger
• Tea
• Arachidonic Acid
• Catechin
• epigallocatechin gallate
• Cyclooxygenase 1
• Cyclooxygenase 2
• PTGS1 protein, human
• PTGS2 protein, human
• Prostaglandin-Endoperoxide Synthases
• Urokinase-Type Plasminogen Activator
• Dinoprostone

Topics

• Antineoplastic Agents (therapeutic use)
• Apoptosis (drug effects)
• Arachidonic Acid (pharmacology)
• Catechin (analogs & derivatives, therapeutic use)
• Cell Division (drug effects)
• Cyclooxygenase 1
• Cyclooxygenase 2
• Dinoprostone (metabolism)
• Humans
• Isoenzymes (antagonists & inhibitors, genetics, metabolism)
• Male
• Membrane Proteins
• Prostaglandin-Endoperoxide Synthases (genetics, metabolism)
• Prostatic Neoplasms (enzymology, genetics, pathology)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tea
• Tumor Cells, Cultured
• Urokinase-Type Plasminogen Activator (antagonists & inhibitors)