Abstract |
Chlamydia are obligate intracellular bacteria that replicate in a vacuole inside a host cell. Chlamydial infection has been shown to protect the host cell against apoptotic stimuli. This is likely important for the ability of Chlamydia to reproduce in human cells. Here we show that resistance to apoptosis is conveyed by the destruction of the proapoptotic BH3-only proteins Bim/Bod, Puma, and Bad during infection. Apoptotic stimuli were blocked upstream of the mitochondrial activation of Bax/Bak. During infection with both species, Chlamydia trachomatis and Chlamydia pneumoniae, Bim protein gradually disappeared without noticeable changes in Bim mRNA. The disappearance was blocked by inhibitors of the proteasome. Infected cells retained sensitivity to Bim expressed by transfection, indicating functional relevance of the Bim disappearance. Fusion to Bim targeted the green fluorescent protein for destruction during infection. Analysis of truncation mutants showed that a short region of Bim containing the BH3 domain was sufficient for destruction during chlamydial infection. Like Bim, Puma and Bad proteins disappeared during infection. These results reveal a novel way by which microbes can interfere with the host cell's apoptotic machinery, and provide a molecular explanation of the cellular resistance to apoptosis during infection with Chlamydia.
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Authors | Silke F Fischer, Juliane Vier, Susanne Kirschnek, Andreas Klos, Simone Hess, Songmin Ying, Georg Häcker |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 200
Issue 7
Pg. 905-16
(Oct 04 2004)
ISSN: 0022-1007 [Print] United States |
PMID | 15452181
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoptosis Regulatory Proteins
- BAD protein, human
- BBC3 protein, human
- BCL2L11 protein, human
- Bax protein (53-86)
- Bcl-2-Like Protein 11
- Carrier Proteins
- Membrane Proteins
- Peptide Fragments
- Proto-Oncogene Proteins
- Tumor Suppressor Protein p53
- bcl-Associated Death Protein
- Green Fluorescent Proteins
- CASP3 protein, human
- Caspase 3
- Caspases
- Proteasome Endopeptidase Complex
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Topics |
- Apoptosis
(immunology, physiology)
- Apoptosis Regulatory Proteins
- Bcl-2-Like Protein 11
- Blotting, Western
- Carrier Proteins
(metabolism)
- Caspase 3
- Caspases
(metabolism)
- Cell Nucleus
(physiology)
- Chlamydia Infections
(immunology, metabolism, physiopathology)
- Flow Cytometry
- Green Fluorescent Proteins
(metabolism)
- Humans
- Membrane Proteins
(metabolism)
- Microscopy, Fluorescence
- Oligonucleotide Array Sequence Analysis
- Peptide Fragments
(metabolism)
- Proteasome Endopeptidase Complex
(metabolism)
- Proto-Oncogene Proteins
(metabolism)
- Transfection
- Tumor Cells, Cultured
- Tumor Suppressor Protein p53
(metabolism)
- bcl-Associated Death Protein
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