Differential expression of the transfected liver-specific alpha 1-inhibitor III gene in normal hepatocytes and hepatoma cells in culture.

Abstract	Normal and malignant hepatocytes were transfected during log phase culture with a nested series of DNA plasmids containing 5'-flanking regions of the rat liver-specific acute phase plasma proteinase alpha 1-inhibitor III (alpha 1 I3) gene. Under these conditions, luciferase reporter gene expression in primary adult rat and mouse hepatocytes was 10-fold higher than luciferase expression in hepatoma lines (human HepG2 and Hep3B; rat FAZA). Optimal expression in primary rat hepatocytes required regions stretching 2214 bp 5'-upstream of the transcription start site. Shorter 5'-flanking sequences were optimal for expression in hepatoma cells (-1025 and -186 bp for rat and human lines, respectively) and primary mouse hepatocytes (-225 bp). In contrast, regions from -186 to -225 bp drove luciferase expression in primary rat hepatocytes, but only 20-75% of optimal levels. Qualitative differences were unaccounted for by non-equivalent uptake of plasmid DNA, suggesting that tissue specific gene expression is regulated differently in normal and malignant cells, and with apparent species specificity.
Authors	K S Koch, X P Lu, D A Brenner, H L Leffert
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 183 Issue 1 Pg. 184-92 (Feb 28 1992) ISSN: 0006-291X [Print] United States
PMID	1543489 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Acute-Phase Proteins Blood Proteins Protease Inhibitors Recombinant Fusion Proteins Transforming Growth Factor alpha alpha 1-inhibitor 3
Topics	Acute-Phase Proteins Animals Blood Proteins (genetics) Cells, Cultured Gene Expression Regulation, Neoplastic (drug effects) Liver (cytology, metabolism) Liver Neoplasms, Experimental (metabolism) Male Protease Inhibitors (metabolism) Rats Recombinant Fusion Proteins Regulatory Sequences, Nucleic Acid (genetics) Transfection Transforming Growth Factor alpha (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!