Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats.

Abstract

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

• Authors: R Zhang, S Barnes, R B Diasio
• Journal: The American journal of physiology (Am J Physiol) Vol. 262 Issue 2 Pt 1 Pg. G351-8 (Feb 1992) ISSN: 0002-9513 [Print] United States
• PMID: 1539668 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bile Acids and Salts
• Chenodeoxycholic Acid
• Taurine
• Glycine

Topics

• Animals
• Bile Acids and Salts (metabolism)
• Chenodeoxycholic Acid (metabolism)
• Glycine (metabolism)
• Intestine, Small (metabolism)
• Male
• Rats
• Rats, Inbred Strains
• Taurine (metabolism)
• Tissue Distribution