Pigment
gallstones are a common clinical complication of sickle cell (SS) disease. Genetic variation in the promoter of
uridine diphosphate (
UDP)-glucuronosyltransferase 1A1 (UGT1A1) underlies
Gilbert syndrome, a chronic form of unconjugated
hyperbilirubinemia, and appears to be a risk factor for
gallstone formation. We investigated the association between UGT1A1 (TA)(n) genotype,
hyperbilirubinemia, and
gallstones in a sample of Jamaicans with SS disease. Subjects were from the Jamaican Sickle Cell Cohort Study (cohort sample, n = 209) and the Sickle Cell Clinic at the University of the West Indies, Kingston, Jamaica (clinic sample, n = 357). The UGT1A1 (TA)(n) promoter region was sequenced in 541 SS disease subjects and 111 healthy controls (control sample). Indirect
bilirubin levels for (TA)(7)/(TA)(7) and (TA)(7)/(TA)(8) genotypes were elevated compared with (TA)(6)/(TA)(6) (clinic sample, P < 10(-5); cohort sample, P < 10(-3)). The (TA)(7)/(TA)(7) genotype was also associated with symptomatic presentation and
gallstones in the clinic sample (odds ratio [OR] = 11.3; P = 7.0 x 10(-4)) but not in the younger cohort sample. These unexpected findings indicate that the temporal evolution of symptomatic
gallstones may involve factors other than the
bilirubin level. Although further studies of the pathogenesis of
gallstones in SS disease are required, the (TA)(7)/(TA)(7) genotype may be a risk factor for symptomatic
gallstones in older people with SS disease.