Abstract |
The protective efficacy of xenogeneic vaccination with DNA encoding the HER2 oncogene was evaluated in BALB/c mice transgenic for the transforming form of the neu oncogene, which spontaneously develops carcinomas in all mammary glands. Intramuscular injection of either plasmid DNA followed by electrical stimulation (pVIJ-HER2 with ES) or an adenoviral vector (Ad5-HER2), both expressing the HER2 oncogene, was tested. Immunization using pVIJ-HER2 with ES elicited a cell-mediated response that was much lower than that elicited by the immunization with Ad5-HER2, as measured by the frequency of IFN-gamma-secreting spleen cells. The dominant T-cell epitope of the HER2 protein product (p185) in the BALB/c (H-2(d)) genetic background was identified. While the T-cell response elicited was only partially crossreactive with the corresponding rat epitopes because of sequence variations (89% similarity), a cytotoxic T-lymphocyte activity against the rat immunodominant epitope was also evident. The Ad5-HER2 vaccination induced also antibodies against p185, which crossreacted with the rat protein homolog. Both T- and B-cell responses slowly declined with time. Vaccination with Ad5-HER2 at 6 and 9 weeks of age delayed incidence and reduced multiplicity of tumors in neu transgenic mice.
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Authors | Pasquale Gallo, Sridhar Dharmapuri, Maurizio Nuzzo, Daniele Maldini, Manuela Iezzi, Federica Cavallo, Piero Musiani, Guido Forni, Paolo Monaci |
Journal | International journal of cancer
(Int J Cancer)
Vol. 113
Issue 1
Pg. 67-77
(Jan 01 2005)
ISSN: 0020-7136 [Print] United States |
PMID | 15386429
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Heterophile
- Cancer Vaccines
- Epitopes, B-Lymphocyte
- Epitopes, T-Lymphocyte
- Immunodominant Epitopes
- Vaccines, DNA
- Interferon-gamma
- Receptor, ErbB-2
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Topics |
- Adenoviridae
(genetics, immunology)
- Animals
- Antibodies, Heterophile
(immunology)
- Cancer Vaccines
(administration & dosage, genetics, immunology)
- Enzyme-Linked Immunosorbent Assay
- Epitopes, B-Lymphocyte
- Epitopes, T-Lymphocyte
- Female
- Flow Cytometry
- Genes, erbB-2
(immunology)
- Genetic Vectors
(genetics, immunology)
- Immunodominant Epitopes
- Interferon-gamma
- Mammary Neoplasms, Experimental
(immunology, prevention & control)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred Strains
- Mice, Transgenic
- Plasmids
(genetics, immunology)
- Rats
- Receptor, ErbB-2
(genetics, immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccines, DNA
(administration & dosage, genetics, immunology)
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