Identification of monovinyl tripropionic acid porphyrins and metabolites from faeces of patients with hereditary coproporphyria by high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.

Abstract

Harderoporphyrin (2-vinyl-4,6,7-tripropionic acid porphyrin) and its metabolites in faeces of patients with hereditary coproporphyria (HCP) have been separated and characterized by high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC/ESI-Q-TOFMS/MS). The metabolites identified were 2-ethyl-4,6,7-tripropionic acid porphyrin, 2-hydro-4,6,7-tripropionic acid porphyrin, 2-methoxyethyl-4,6,7-tripropionic acid porphyrin and 2-acetyl-4,6,7-tripropionic acid porphyrin. Isomers of harderoporphyrin derived from isomerization of harderoporphyrinogen were also detected.

• Authors: Malcolm Danton, Chang Kee Lim
• Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom) Vol. 18 Issue 19 Pg. 2309-16 ( 2004) ISSN: 0951-4198 [Print] England
• PMID: 15384152 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Validation Study)
• Copyright: Copyright 2004 John Wiley & Sons, Ltd.

Chemical References

• Porphyrins
• harderoporphyrin

Topics

• Chromatography, High Pressure Liquid (methods)
• Coproporphyria, Hereditary (diagnosis, metabolism)
• Feces (chemistry)
• Humans
• Porphyrins (analysis, classification)
• Reproducibility of Results
• Sensitivity and Specificity
• Spectrometry, Mass, Electrospray Ionization (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)