Enhancement of sodium borocaptate (BSH) uptake by tumor cells induced by glutathione depletion and its radiobiological effect.

Abstract

Sodium borocaptate (BSH) is widely used for boron neutron capture therapy (BNCT) of brain tumors. However, the mechanism of uptake by the tumor remains unclear. We investigated the sulfhydryl moiety of this compound. Down regulation of glutathione (GSH) by buthionine sulfoximine in cultured cells resulted in increase of BSH uptake (7.9-36.5%) compared to the control group and consequently the cytocidal effect of neutron irradiation also increased. On the other hand, the radiation caused damage by gamma-ray irradiation was suppressed when BSH uptake increased. These findings suggested that modulation of GSH enhanced the effect of B (n, alpha) reaction and the protective effect of secondary gamma-ray in BNCT.

• Authors: Fumiyo Yoshida, Akira Matsumura, Tetsuya Yamamoto, Hiroaki Kumada, Kei Nakai
• Journal: Cancer letters (Cancer Lett) Vol. 215 Issue 1 Pg. 61-7 (Nov 08 2004) ISSN: 0304-3835 [Print] Ireland
• PMID: 15374633 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Borohydrides
• Radiation-Sensitizing Agents
• Sulfhydryl Compounds
• mercaptoundecahydrododecaborate
• Buthionine Sulfoximine
• Glutathione

Topics

• Animals
• Borohydrides (metabolism)
• Boron Neutron Capture Therapy
• Brain Neoplasms (metabolism, radiotherapy)
• Buthionine Sulfoximine (pharmacology)
• Carcinoma, Squamous Cell (metabolism, radiotherapy)
• Cells, Cultured
• Cricetinae
• Down-Regulation
• Gamma Rays
• Glioma (metabolism, radiotherapy)
• Glutathione (metabolism)
• Lung (cytology, drug effects)
• Mice
• Radiation-Sensitizing Agents (pharmacology)
• Rats
• Relative Biological Effectiveness
• Sulfhydryl Compounds (metabolism)