| Abstract | Macrophages co-incubated with Candida albicans strain CR1 in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CR1 caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL-10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat-killed C. albicans did not cause those effects. IL-10 production was low during the first 6 h post-infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CR1 with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL-10 production, suggesting that the increased production of IL-10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells. |
| Authors | Thaís Helena Gasparoto, Luis Carlos Jabur Gaziri, Eva Burger, Ricardo Sérgio Couto de Almeida, Ionice Felipe
(Affiliation: Departamento de Ciências Patológicas, CCB, Universidade Estadual de Londrina, Cx P. 6001, 86051-990 Londrina, Brazil.)
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| Journal | FEMS immunology and medical microbiology
(FEMS Immunol Med Microbiol)
Vol. 42
Issue 2
Pg. 219-24
(Oct 1 2004)
ISSN: 0928-8244 Netherlands |
| PMID | 15364107
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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| Chemical References |
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| Topics |
- Animals
- Apoptosis
- Candida albicans
(pathogenicity)
- Candidiasis
(immunology, microbiology)
- Interleukin-10
(biosynthesis)
- Macrophages, Peritoneal
(immunology, microbiology, physiology)
- Male
- Mice
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