Abstract |
Two enzyme-linked immunosorbent assay (ELISA) systems, one using natural excretory-secretory (ES) antigens and the other a synthetic glycan antigen (3,6-dideoxy-D-arabinohexose [ tyvelose, TY]), were evaluated for the serological diagnosis of trichinellosis in swine. Sensitivity was estimated using samples (n = 113) collected 3-21 wk PI from 15 experimentally infected pigs, and specificity was estimated using samples (n = 397) from a population of Trichinella spp.-free pigs. Results were analyzed using 2 cutoff values recommended in international guidelines (Office Internationale des Epizooties [OIE]) and by the optimal cutoff level as determined by receiver-operator characteristic (ROC) analysis. The ROC-optimized TY-ELISA consistently performed better than all other combinations. None of the combinations of test and cut-off detected infected pigs sooner than 35 days; however, the ROC-optimized TY-ELISA identified 8 of 15 pigs earlier than the ES-ELISA and detected 2 pigs missed by all other tests. At 49 days PI the sensitivity and specificity of the ROC-optimized TY-ELISA were 94.3 and 96.7%, respectively, as compared with the ROC-optimized ES-ELISA at 84.9 and 96.0%, respectively. The ROC-optimized TY-ELISA was 100% specific at OIE-recommended cut-offs. This study indicates that the TY-ELISA is as good or better than the ES-ELISA for the detection of trichinellosis in swine.
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Authors | Lorry B Forbes, Greg D Appleyard, Alvin A Gajadhar |
Journal | The Journal of parasitology
(J Parasitol)
Vol. 90
Issue 4
Pg. 835-40
(Aug 2004)
ISSN: 0022-3395 [Print] United States |
PMID | 15357078
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Hexoses
- tyvelose
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Topics |
- Animals
- Antibodies, Helminth
(blood)
- Antigens, Helminth
(immunology)
- Enzyme-Linked Immunosorbent Assay
(methods, veterinary)
- Female
- Hexoses
(chemical synthesis, immunology)
- ROC Curve
- Sensitivity and Specificity
- Swine
- Swine Diseases
(diagnosis, immunology)
- Trichinella
(immunology)
- Trichinellosis
(diagnosis, immunology, veterinary)
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