Abstract |
Increased oxidative stress induced by hyperglycemia may contribute to the pathogenesis of diabetic complications. Urinary 8-hydroxydeoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. This article studied oxidative DNA damage in patients with diabetic nephropathy and in healthy control subjects by urinary 8-OHdG evaluations. Contents of 8-OHdG in urine were analyzed by capillary electrophoresis with end-column amperometric detection (CE-AD) after a single-step solid-phase extraction (SPE). Levels of urinary 8-OHdG in diabetic nephropathy patients with macroalbuminuria was significant higher than in control subjects (5.72 +/- 6.89 micromol/mol creatinine versus 2.33 +/- 2.83 micromol/mol creatinine, P = 0.018). A significant difference of 24 h urinary 8-OHdG excretions exists between the patients with macroalbuminuria and the patients with normoalbuminuria (19.2 +/- 16.8 microg/24 h versus 8.1 +/- 1.7 microg/24 h, P = 0.015). There was a positive correlation between urinary excretion of 8-OHdG and glycosylated hemoglobin (HbA1c) (r = 0.287, P = 0.022). A weak correlation exists between the levels of 8-OHdG and triglyceride (r = 0.230, P = 0.074). However, the urinary 8-OHdG contents are not correlated with blood pressure and total cholesterol. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in diabetic nephropathy patients.
|
Authors | G W Xu, Q H Yao, Q F Weng, B L Su, X Zhang, J H Xiong |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 36
Issue 1
Pg. 101-4
(Sep 21 2004)
ISSN: 0731-7085 [Print] England |
PMID | 15351053
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Biomarkers
- 8-Hydroxy-2'-Deoxyguanosine
- Deoxyguanosine
|
Topics |
- 8-Hydroxy-2'-Deoxyguanosine
- Adult
- Aged
- Aged, 80 and over
- Biomarkers
(urine)
- DNA Damage
- Deoxyguanosine
(analogs & derivatives, urine)
- Diabetes Mellitus, Type 2
(complications, urine)
- Diabetic Nephropathies
(etiology, urine)
- Electrophoresis, Capillary
- Female
- Humans
- Male
- Middle Aged
|