Cloning and identification of a novel human gene PDLIM5, a homolog of AD-associated neuronal thread protein (AD7c-NTP).

Abstract

A novel human gene cDNA was successfully cloned from the human fetal brain cDNA library constructed by our lab, and this gene was termed PDLIM5 after acquiring the agreement of HUGO. BLASTX searching revealed that the hypothetical protein is a homolog of AD-associated neuronal thread protein (AD7c-NTP), which is over-expressed in Alzheimer disease (AD) beginning early in the course of disease, and over-expression of the AD7c-NTP gene would cause neuritic sprouting and cell death. SMART analysis showed that both our predicted protein and AD7c-NTP comprise BCL domain (only contains BH1 and BH2 regions). RT-PCR experiment revealed that the expression level of PDLIM5 in brain, skeletal muscle, prostate, colon and leukocyte is obviously higher than that in other tissues.

• Authors: Maoqing Wu, Yao Li, Chaoneng Ji, Jian Xu, Huarui Zheng, Xianqiong Zou, Shaohua Gu, Yi Lou, Yi Xie, Yumin Mao
• Journal: DNA sequence : the journal of DNA sequencing and mapping (DNA Seq) Vol. 15 Issue 2 Pg. 144-7 (Apr 2004) ISSN: 1042-5179 [Print] England
• PMID: 15346770 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AD7c-NTP
• DNA, Complementary
• Nerve Tissue Proteins

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (analysis)
• Humans
• Molecular Sequence Data
• Nerve Tissue Proteins (genetics)
• Protein Structure, Tertiary
• Sequence Homology, Amino Acid