Abstract |
Alteration of the cell surface glycoproteins of cancerous cells correlate with malignancy potential. To evaluate the mediation of membrane glycoproteins in wehi-164 cancerous cells, under the effect of D. mucronata crude extract and one of its purified active components, gnidilatimonoein, their attachment to fibronectin-coated wells were investigated. The plant extract, 27 microl/ml (equivalent to 0.54 mg/of plant leaves powder per ml of culture medium), as well as gnidilatimonoein (0.94 microM), were capable of quenching by 58% and 64%, respectively, the attachment of wehi-164 cells to fibronectin-coated wells (4 microg/ml). In addition to alteration of cell adhesive properties, the morphology of the treated cells were significantly changed upon treatment with the non-toxic dose of the plant extract or gnidilatimonoein. While the untreated cells have polygonal shapes, the treated cells had spherical shapes.
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Authors | M Mianabadi, R Yazdanparast |
Journal | The American journal of Chinese medicine
(Am J Chin Med)
Vol. 32
Issue 3
Pg. 369-76
( 2004)
ISSN: 0192-415X [Print] Singapore |
PMID | 15344420
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Diterpenes
- Fibronectins
- Plant Extracts
- gnidilatimonoein
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Topics |
- Animals
- Cell Adhesion
(drug effects)
- Cell Line, Tumor
(cytology, drug effects)
- Daphne
- Diterpenes
(pharmacology)
- Fibronectins
(drug effects, metabolism)
- Plant Extracts
(pharmacology)
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