Vitamin A reduces lung granulomatous inflammation with eosinophilic and neutrophilic infiltration in Sephadex-treated rats.

Abstract

Vitamin A is known to suppress the activity of the transcription factors, nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1), as do glucocorticoids. The possibility that vitamin A exerts various anti-inflammatory effects therefore seems likely. Sephadex beads were administered intravenously to anesthesized rats pretreated with a subcutaneous injection of vitamin A (3000, 10,000, or 30,000 IU/kg) or vehicle once daily for 3 days. After 16 h, the leukocyte differential, tumor necrosis factor (TNF)-alpha and eotaxin, and the DNA-binding activity of NF-kappaB were measured in bronchoalveolar lavage fluid (BALF). Additionally, lung histology was assessed using preparations stained with May-Giemsa stain. Sephadex beads caused histological granulomatous changes and eosinophilic and neutrophilic infiltration into the lung, and markedly increased cell counts of eosinophils and neutrophils, concentrations of TNF-alpha and eotaxin, and NF-kappaB binding to DNA in BALF. Vitamin A significantly inhibited all responses. Vitamin A may inhibit Sephadex-induced lung granulomatous formation, and eosinophilic and neutrophilic infiltration due to its suppression of TNF-alpha and eotaxin production, and NF-kappaB activation.

• Authors: Akiko Torii, Mio Miyake, Masashi Morishita, Komei Ito, Shinpei Torii, Tatsuo Sakamoto
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 497 Issue 3 Pg. 335-42 (Aug 30 2004) ISSN: 0014-2999 [Print] Netherlands
• PMID: 15336952 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Ccl11 protein, rat
• Cell Extracts
• Chemokine CCL11
• Chemokines, CC
• Cytokines
• DNA-Binding Proteins
• Dextrans
• NF-kappa B
• Tumor Necrosis Factor-alpha
• Vitamin A
• sephadex

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, pharmacology, therapeutic use)
• Bronchoalveolar Lavage Fluid (chemistry)
• Cell Extracts (chemistry)
• Chemokine CCL11
• Chemokines, CC (biosynthesis)
• Cytokines (metabolism)
• DNA-Binding Proteins (metabolism)
• Dextrans
• Dose-Response Relationship, Drug
• Eosinophils (pathology)
• Granuloma, Respiratory Tract (drug therapy, etiology, pathology)
• Injections, Subcutaneous
• Leukocytes (drug effects, metabolism)
• Lung (metabolism, pathology)
• Male
• NF-kappa B (metabolism)
• Neutrophil Infiltration
• Pneumonia (drug therapy, etiology, pathology)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (metabolism)
• Vitamin A (administration & dosage, pharmacology, therapeutic use)