Abstract | BACKGROUND: MATERIALS AND METHODS: Four gastric cancer cell lines, OCUM-2M, MKN-28, MKN-45 and MKN-74, and two fibroblast cell lines were used in this study. The proliferation of gastric cancer cells was determined by MTT assay, by calculating the number of cancer cells, and by [3H]- thymidine uptake. Cells were cultured in DMEM containing 10% FBS with glucose (4.5 g/L) as the control or with MAA (4.5 g/L). SCOT mRNA expression was examined by RT-PCR. RESULTS: The growth of OCUM-2M and MKN-28 cells was significantly suppressed in MAA medium compared with glucose medium. In contrast the growth of MKN-74, MKN-45 and normal fibroblasts was not suppressed in MAA medium. SCOT mRNA was expressed in MKN-45, MKN-74 and normal fibroblasts, but not in MKN-28 or OCUM-2M. CONCLUSION:
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Authors | Masamitsu Sawai, Masakazu Yashiro, Yukio Nishiguchi, Masaichi Ohira, Kosei Hirakawa |
Journal | Anticancer research
(Anticancer Res)
2004 Jul-Aug
Vol. 24
Issue 4
Pg. 2213-7
ISSN: 0250-7005 [Print] Greece |
PMID | 15330163
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acetoacetates
- Glycerides
- Growth Inhibitors
- RNA, Messenger
- monoacetoacetin
- Coenzyme A-Transferases
- 3-ketoacid CoA-transferase
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Topics |
- Acetoacetates
(pharmacology)
- Cell Division
(drug effects)
- Cell Line, Tumor
- Coenzyme A-Transferases
(biosynthesis, deficiency, genetics)
- Glycerides
(pharmacology)
- Growth Inhibitors
(pharmacology)
- Humans
- RNA, Messenger
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Stomach Neoplasms
(drug therapy, enzymology, genetics, pathology)
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