HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Analysis of interleukin-13 receptor alpha2 expression in human pediatric brain tumors.

AbstractBACKGROUND:
Compared with normal brain tissue cells, human malignant glioma cells express higher levels of interleukin-13 receptor (IL-13R). However, whether this receptor is expressed in situ has not been carefully examined. With IL-13R-targeted cytotoxin (IL13-PE38QQR, comprising IL-13 and a mutated form of Pseudomonas exotoxin [PE]) being tested in three Phase I/II clinical trials for the treatment of adult human glioma, and with pediatric studies being planned, the authors set out to analyze pediatric brain tumor tissue specimens for the expression of IL-13R.
METHODS:
Using in situ hybridization and immunohistochemical staining, the authors examined 58 pediatric brain tumor specimens for expression of the predominant IL-13 binding and internalizing protein (IL-13Ralpha2) chain at the mRNA and protein levels.
RESULTS:
Overall, approximately 83% of pediatric brain tumor samples expressed IL-13Ralpha2. One hundred percent (11 of 11) high-grade astrocytoma, 79% (26 of 33) low-grade astrocytoma, 67% (4 of 6) medulloblastoma, and 67% (2 of 3) ependymoma samples were positive for IL-13Ralpha2. Among IL-13Ralpha2-positive samples, 88% (42 of 48 samples) had positive expression in > or = 50% of all tumor fields. The results obtained using both assays were consistent with each other.
CONCLUSIONS:
The current study established that pediatric brain tumor specimens expressed the IL-13Ralpha2 chain. Because the IL-13Ralpha2 chain is a major binding component of the IL-13R complex, these results suggest that the targeting of IL-13R may represent a useful approach for the treatment of pediatric brain tumors.
AuthorsMariko Kawakami, Koji Kawakami, Satoru Takahashi, Masato Abe, Raj K Puri
JournalCancer (Cancer) Vol. 101 Issue 5 Pg. 1036-42 (Sep 01 2004) ISSN: 0008-543X [Print] United States
PMID15329913 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
CopyrightCopyright 2004 American Cancer Society.
Chemical References
  • IL13RA1 protein, human
  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • RNA Probes
  • Receptors, Interleukin
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4
Topics
  • Adolescent
  • Adult
  • Astrocytoma (metabolism, pathology)
  • Brain Neoplasms (metabolism, pathology)
  • Child
  • Child, Preschool
  • Ependymoma (metabolism, pathology)
  • Female
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Infant
  • Interleukin-13 (metabolism)
  • Interleukin-13 Receptor alpha1 Subunit
  • Male
  • Medulloblastoma (metabolism, pathology)
  • RNA Probes
  • Receptors, Interleukin (metabolism)
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4 (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: