Abstract | BACKGROUND: Antiendothelial cell antibodies ( AECA), usually detected using human umbilical vein endothelial cells (HUVEC), are frequently observed in systemic vasculitis, but their pathogenic role is unclear. Heterogeneity of endothelial cells necessitates use of clinically relevant endothelial cells for elucidation of the role of AECA in systemic vasculitis involving small blood vessels of specific organs. METHODS: Human endothelial cells were isolated from normal tissue specimens from the nose, kidney, lung, liver, and umbilical vein. Using flow cytometry, AECA were detected against both unstimulated and cytokine-stimulated [ tumor necrosis factor-alpha ( TNF-alpha) and interferon-gamma (IFN-gamma)] endothelial cells. Functional capacity of AECA was determined by complement fixation assay. Sera from patients with Wegener's granulomatosis (16), limited Wegener's granulomatosis (8), renal limited disease (4), microscopic polyangiitis (MPA) (5), rheumatoid arthritis (10), and systemic lupus erythematosus (SLE) (9), and from healthy controls (20) were analyzed. RESULTS: CONCLUSION:
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Authors | Carolina Holmén, Marta Christensson, Erna Pettersson, Johan Bratt, Pär Stjärne, Azza Karrar, Suchitra Sumitran-Holgersson |
Journal | Kidney international
(Kidney Int)
Vol. 66
Issue 3
Pg. 1049-60
(Sep 2004)
ISSN: 0085-2538 [Print] United States |
PMID | 15327398
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Tumor Necrosis Factor-alpha
- anti-endothelial cell antibody
- Interferon-gamma
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Topics |
- Autoantibodies
(immunology)
- Complement Fixation Tests
- Endothelial Cells
(drug effects, immunology)
- Endothelium, Vascular
(cytology, immunology)
- Granulomatosis with Polyangiitis
(immunology)
- Humans
- Immunophenotyping
- Interferon-gamma
(pharmacology)
- Kidney
(cytology, immunology)
- Lung
(cytology, immunology)
- Nose
(cytology, immunology)
- Organ Specificity
- Tumor Necrosis Factor-alpha
(pharmacology)
- Umbilical Veins
(cytology)
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