Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP.

Abstract

Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.

• Authors: Beata Kosmider, Elzbieta Zyner, Regina Osiecka, Justyn Ochocki
• Journal: Mutation research (Mutat Res) Vol. 563 Issue 1 Pg. 61-70 (Sep 12 2004) ISSN: 0027-5107 [Print] Netherlands
• PMID: 15324749 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3-aminoflavone
• Antineoplastic Agents
• Flavonoids
• Fluorescent Dyes
• Tetrazolium Salts
• Thiazoles
• Ethidium
• thiazolyl blue
• Acridine Orange
• Cisplatin

Topics

• Acridine Orange (metabolism)
• Antineoplastic Agents (chemistry, toxicity)
• Apoptosis
• Cell Division
• Cell Line, Tumor
• Cell Survival
• Cisplatin (chemistry, metabolism, toxicity)
• Ethidium (metabolism)
• Flavonoids (chemistry, metabolism)
• Fluorescent Dyes (metabolism)
• Humans
• In Situ Nick-End Labeling
• Molecular Structure
• Necrosis
• Tetrazolium Salts (metabolism)
• Thiazoles (metabolism)