Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection.

Abstract

Up to a third of autologous transplantation candidates fail to mobilize hematopoietic progenitors into the peripheral blood with chemotherapy and/or growth factor treatment, thus requiring innovative mobilization strategies. In total, 20 cancer patients unable to provide adequate PBPC products after a previous mobilization attempt were treated with ancestim (20 microg/kg/day s.c.) and filgrastim (10 microg/kg/day s.c.). In 16 patients, the pre-study mobilization was with filgrastim alone. Eight patients underwent single large volume leukapheresis (LVL) and 12 multiple standard volume leukaphereses (SVL) in both mobilizations. Pairwise comparison of peripheral blood CD34(+) cell concentrations on the day of first leukapheresis failed to document synergism - median CD34(+)/microl of 3.2 (<0.1 to 15.4) and 4.5 (1-28.56) for the pre-study and on-study mobilizations (P = 0.79, sign test), and 4.2 (<0.1-15.4) and 5 (1-28.56), respectively, for the 16 patients previously mobilized with filgrastim alone (P = 1, sign test). The number of CD34(+) cells/kg collected per unit of blood volume (BV) processed was similar in both mobilizations - median 0.1 x 10(6)/kg/BV and 0.09 x 10(6)/kg/BV, respectively (P = 1, sign test). In this phase II study, the combination of ancestim and filgrastim did not allow adequate PBPC mobilization and collection in patients with a previous suboptimal PBPC collection.

• Authors: M G da Silva, P Pimentel, A Carvalhais, I Barbosa, A Machado, F Campilho, S R Sousa, N Miranda, F Leal da Costa, A Campos, C P Vaz, J Antas, J L Passos-Coelho
• Journal: Bone marrow transplantation (Bone Marrow Transplant) Vol. 34 Issue 8 Pg. 683-91 (Oct 2004) ISSN: 0268-3369 [Print] England
• PMID: 15322567 (Publication Type: Journal Article)

Chemical References

• Antigens, CD34
• Recombinant Proteins
• Stem Cell Factor
• Granulocyte Colony-Stimulating Factor
• Filgrastim
• ancestim

Topics

• Adult
• Antigens, CD34 (biosynthesis)
• Female
• Filgrastim
• Granulocyte Colony-Stimulating Factor (chemistry, metabolism)
• Hematopoietic Stem Cell Mobilization (methods)
• Humans
• Leukapheresis (methods)
• Leukocytes, Mononuclear (cytology)
• Male
• Middle Aged
• Recombinant Proteins
• Stem Cell Factor (analogs & derivatives, chemistry, metabolism)
• Stem Cells (cytology)
• Time Factors
• Transplantation, Autologous