Postsynaptic density protein 95 antisense oligodeoxynucleotides inhibits the activation of MLK3 and JNK3 via the GluR6.PSD-95.MLK3 signaling module after transient cerebral ischemia in rat hippocampus.

Abstract

In this study, we investigated the effect of PSD-95 antisense oligodeoxynucleotides on the phosphorylation of MLK3, JNK3 and interactions of MLK3 and PSD-95 with kainate receptor (GluR6) by immunoprecipitation and immunoblotting. Transient (15 min) brain ischemia was induced by the four-vessel occlusion in Sprague-Dawley rats. The antisense oligodeoxynucleotides of PSD-95 were administrated to the SD rats once per day for 3 days before ischemia. Our data show that the antisense oligodeoxynucleotides could inhibit phosphorylation of MLK3 and JNK3 and decrease the interactions of MLK3 and PSD-95 with GluR6. These results indicate that PSD-95 plays an important role in the formation of the GluR6.PSD-95.MLK3 signaling module and MLK3 and JNK3 activation in postischemic rat hippocampus.

• Authors: Dong-Sheng Pei, Ya-Feng Sun, Qiu-Hua Guan, Zhi-Bin Hao, Tian-Le Xu, Guang-Yi Zhang
• Journal: Neuroscience letters (Neurosci Lett) Vol. 367 Issue 1 Pg. 71-5 (Aug 26 2004) ISSN: 0304-3940 [Print] Ireland
• PMID: 15308300 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Disks Large Homolog 4 Protein
• Dlg4 protein, rat
• Gluk2 kainate receptor
• Intracellular Signaling Peptides and Proteins
• Membrane Proteins
• Nerve Tissue Proteins
• Oligodeoxyribonucleotides, Antisense
• Receptors, Kainic Acid
• postsynaptic density proteins
• Mitogen-Activated Protein Kinase 10
• MAP Kinase Kinase Kinases
• mitogen-activated protein kinase kinase kinase 11
• Mitogen-Activated Protein Kinase Kinases

Topics

• Animals
• Blotting, Western (methods)
• Disks Large Homolog 4 Protein
• Hippocampus (drug effects, metabolism)
• Immunoprecipitation (methods)
• Intracellular Signaling Peptides and Proteins
• Ischemic Attack, Transient (metabolism, prevention & control)
• MAP Kinase Kinase Kinases
• Male
• Membrane Proteins
• Mitogen-Activated Protein Kinase 10 (metabolism)
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Nerve Tissue Proteins (chemistry, metabolism)
• Oligodeoxyribonucleotides, Antisense (pharmacology, therapeutic use)
• Phosphorylation (drug effects)
• Rats
• Rats, Sprague-Dawley
• Receptors, Kainic Acid (physiology)
• Signal Transduction (drug effects)