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Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.

Abstract
Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
AuthorsMaria Cecilia Huaman, Yoshiya Sato, Jose Luis Aguilar, Angelica Terashima, Humberto Guerra, Eduardo Gotuzzo, Hiroji Kanbara
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg) 2003 Sep-Oct Vol. 97 Issue 5 Pg. 535-8 ISSN: 0035-9203 [Print] England
PMID15307419 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Helminth
Topics
  • Adolescent
  • Adult
  • Aged
  • Agglutination Tests (methods, standards)
  • Analysis of Variance
  • Animals
  • Antigens, Helminth (analysis)
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay (methods, standards)
  • HTLV-I Infections (complications)
  • Humans
  • Middle Aged
  • Sensitivity and Specificity
  • Strongyloides (immunology, isolation & purification)
  • Strongyloidiasis (diagnosis)

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