Abstract |
Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination ( GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
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Authors | Maria Cecilia Huaman, Yoshiya Sato, Jose Luis Aguilar, Angelica Terashima, Humberto Guerra, Eduardo Gotuzzo, Hiroji Kanbara |
Journal | Transactions of the Royal Society of Tropical Medicine and Hygiene
(Trans R Soc Trop Med Hyg)
2003 Sep-Oct
Vol. 97
Issue 5
Pg. 535-8
ISSN: 0035-9203 [Print] England |
PMID | 15307419
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Agglutination Tests
(methods, standards)
- Analysis of Variance
- Animals
- Antigens, Helminth
(analysis)
- Child
- Child, Preschool
- Enzyme-Linked Immunosorbent Assay
(methods, standards)
- HTLV-I Infections
(complications)
- Humans
- Middle Aged
- Sensitivity and Specificity
- Strongyloides
(immunology, isolation & purification)
- Strongyloidiasis
(diagnosis)
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