Lithospermic acid B isolated from Salvia miltiorrhiza ameliorates ischemia/reperfusion-induced renal injury in rats.

Abstract

The present study was designed to examine whether lithospermic acid B (LSB) isolated from Salvia miltiorrhiza has an ameliorative effect on renal functional parameters in association with the expression of aquaporin 2 (AQP 2) and Na,K-ATPase in the ischemia-reperfusion induced acute renal failure (ARF) rats. LSB showed strong antioxidant activity against production of reactive oxygen species (ROS), ROS-induced hemolysis, and production of lipid peroxide in a dose-dependent manner. Polyuria caused by down-regulation of renal AQP 2 in the ischemia-reperfusion induced ARF rats was partially restored by administration of LSB (40 mg/kg, i.p.), restoring expression of AQP 2, in renal inner and outer medulla. The expression of Na,K-ATPase alpha1 subunit in outer medulla of the ARF rats was also restored in the ARF rats by administration of LSB, while beta1 subunit level was not altered. The renal functional parameters including creatinine clearance, urinary sodium excretion, urinary osmolality, and solute-free reabsorption were also partially restored in ischemia-ARF rats by administration of LSB. Histological study also showed that renal damages in the ARF rats were abrogated by administration of LSB. Taken together, these data indicate that LSB ameliorates renal defects in rats with ischemia-reperfusion induced ARF, most likely via scavenging of ROS.

• Authors: Dae Gill Kang, Hyuncheol Oh, Eun Jin Sohn, Tae Young Hur, Kang Chang Lee, Kwang Jin Kim, Tai Yo Kim, Ho Sub Lee
• Journal: Life sciences (Life Sci) Vol. 75 Issue 15 Pg. 1801-16 (Aug 27 2004) ISSN: 0024-3205 [Print] Netherlands
• PMID: 15302225 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aqp2 protein, rat
• Aquaporin 2
• Aquaporins
• Benzofurans
• Depsides
• Enzyme Inhibitors
• Free Radical Scavengers
• Oxidants
• Proteins
• Superoxides
• Hydroxyl Radical
• salvianolic acid B
• Sodium-Potassium-Exchanging ATPase

Topics

• Acute Kidney Injury (drug therapy, etiology, pathology)
• Animals
• Aquaporin 2
• Aquaporins (antagonists & inhibitors, biosynthesis)
• Benzofurans (isolation & purification, pharmacology)
• Blotting, Western
• Depsides
• Enzyme Inhibitors (isolation & purification, pharmacology)
• Erythrocytes (drug effects)
• Free Radical Scavengers (pharmacology)
• Hemolysis (drug effects)
• Hydroxyl Radical (metabolism)
• In Vitro Techniques
• Kidney (metabolism, pathology)
• Kidney Function Tests
• Lipid Peroxidation (drug effects)
• Male
• Oxidants (metabolism)
• Plant Roots (chemistry)
• Proteins (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy)
• Salvia miltiorrhiza (chemistry)
• Sodium-Potassium-Exchanging ATPase (antagonists & inhibitors, biosynthesis)
• Superoxides (metabolism)