Abstract | PURPOSE: METHODS: Twenty-five tear film and eye swab specimens from 25 herpetic keratitis patients and 10 specimens from 10 healthy volunteers were collected in the Farabi Eye Hospital, Tehran, Iran. HSV-1 DNA was detected by using the nested polymerase chain reaction (nPCR) method. Viral isolation was done using conventional viral techniques. A monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was used for confirmation of positive cytopathic effect cell culture. The results of a diagnosis by an ophthalmologist team were compared with those of nPCR. RESULTS: HSV-1 DNA was identified in tear films of 88% (23/25) of suspected herpetic keratitis patients. All healthy controls (100%) had negative PCR results. HSV-1 was isolated in cell culture and confirmed by ELISA in 12% (3/25) of herpetic keratitis patients who had epithelial keratitis. The kappa value showed a high level of agreement between ophthalmologist team diagnosis and the PCR results (kappa = 0.86, P < 0.0001). CONCLUSIONS:
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Authors | Mohammad Ali Khodadoost, Farzaneh Sabahi, Mahmoud Jabbarvand Behroz, Mohammad Hassan Roustai, Horieh Saderi, Samad Amini-Bavil-Olyaee, Mohsen Karimi Arzenani |
Journal | Japanese journal of ophthalmology
(Jpn J Ophthalmol)
2004 Jul-Aug
Vol. 48
Issue 4
Pg. 328-32
ISSN: 0021-5155 [Print] Japan |
PMID | 15295656
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright Japanese Ophthalmological Society 2004 |
Chemical References |
- Antibodies, Viral
- DNA, Viral
- Viral Envelope Proteins
- glycoprotein D, Human herpesvirus 1
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Topics |
- Animals
- Antibodies, Viral
(analysis)
- Chlorocebus aethiops
- Cytopathogenic Effect, Viral
- DNA, Viral
(analysis)
- Enzyme-Linked Immunosorbent Assay
- Female
- HeLa Cells
- Herpesvirus 1, Human
(genetics, immunology, isolation & purification)
- Humans
- Iran
- Keratitis, Herpetic
(virology)
- Male
- Polymerase Chain Reaction
(methods)
- Tears
(virology)
- Vero Cells
- Viral Envelope Proteins
(genetics)
- Virus Cultivation
(methods)
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