HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Study of a polymerase chain reaction-based method for detection of herpes simplex virus type 1 DNA among Iranian patients with ocular herpetic keratitis infection.

AbstractPURPOSE:
To study the presence of the herpes simplex virus type 1 (HSV-1) glycoprotein D gene in tear films of Iranian patients with herpetic keratitis.
METHODS:
Twenty-five tear film and eye swab specimens from 25 herpetic keratitis patients and 10 specimens from 10 healthy volunteers were collected in the Farabi Eye Hospital, Tehran, Iran. HSV-1 DNA was detected by using the nested polymerase chain reaction (nPCR) method. Viral isolation was done using conventional viral techniques. A monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was used for confirmation of positive cytopathic effect cell culture. The results of a diagnosis by an ophthalmologist team were compared with those of nPCR.
RESULTS:
HSV-1 DNA was identified in tear films of 88% (23/25) of suspected herpetic keratitis patients. All healthy controls (100%) had negative PCR results. HSV-1 was isolated in cell culture and confirmed by ELISA in 12% (3/25) of herpetic keratitis patients who had epithelial keratitis. The kappa value showed a high level of agreement between ophthalmologist team diagnosis and the PCR results (kappa = 0.86, P < 0.0001).
CONCLUSIONS:
nPCR is a sensitive, rapid, and powerful tool for detection of HSV-1 DNA in tear films of ocular herpetic keratitis patients and can serve as a supplemental method for diagnosis of herpetic keratitis infection.
AuthorsMohammad Ali Khodadoost, Farzaneh Sabahi, Mahmoud Jabbarvand Behroz, Mohammad Hassan Roustai, Horieh Saderi, Samad Amini-Bavil-Olyaee, Mohsen Karimi Arzenani
JournalJapanese journal of ophthalmology (Jpn J Ophthalmol) 2004 Jul-Aug Vol. 48 Issue 4 Pg. 328-32 ISSN: 0021-5155 [Print] Japan
PMID15295656 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright Japanese Ophthalmological Society 2004
Chemical References
  • Antibodies, Viral
  • DNA, Viral
  • Viral Envelope Proteins
  • glycoprotein D, Human herpesvirus 1
Topics
  • Animals
  • Antibodies, Viral (analysis)
  • Chlorocebus aethiops
  • Cytopathogenic Effect, Viral
  • DNA, Viral (analysis)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • HeLa Cells
  • Herpesvirus 1, Human (genetics, immunology, isolation & purification)
  • Humans
  • Iran
  • Keratitis, Herpetic (virology)
  • Male
  • Polymerase Chain Reaction (methods)
  • Tears (virology)
  • Vero Cells
  • Viral Envelope Proteins (genetics)
  • Virus Cultivation (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: