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Evaluation of autoimmunity to transaldolase in multiple sclerosis.

Abstract
Transaldolase is a target of autoimmunity mediated by T cells and antibody (Ab) in patients with multiple sclerosis. Functional T-cell assays, T- and B-cell epitope mapping, and detection of transaldolase-specific antibodies in patients with multiple sclerosis are described. Recombinant transaldolase was produced in a prokaryotic expression vector for use in Western blot analysis of sera of these patients. Overlapping transaldolase peptides 15 amino acids (aa) long were synthesized onto cellulose membranes to map immunodominant B-cell epitopes. Amino acid sequence homologies between viral peptides and immunodominant B-cell epitopes of transaldolase were identified using a computer-based algorithm. Direct assessment of molecular mimicry between transaldolase B-cell epitopes and related viral peptides is also shown. T-cell epitopes are mapped in a T-cell proliferation assay using multiple sclerosis patient and control donor cells. Autoantigen-specific T cells are identified by MHC-peptide tetramer staining using flow cytometry analysis.
AuthorsBrian Niland, Andras Perl
JournalMethods in molecular medicine (Methods Mol Med) Vol. 102 Pg. 155-71 ( 2004) ISSN: 1543-1894 [Print] United States
PMID15286385 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Autoantibodies
  • Autoantigens
  • Immunodominant Epitopes
  • Transaldolase
Topics
  • Amino Acid Sequence
  • Autoantibodies (blood)
  • Autoantigens
  • Autoimmunity
  • B-Lymphocytes (immunology)
  • Epitope Mapping
  • Flow Cytometry
  • Humans
  • Immunodominant Epitopes (genetics)
  • Molecular Mimicry
  • Molecular Sequence Data
  • Multiple Sclerosis (enzymology, genetics, immunology)
  • Sequence Homology, Amino Acid
  • T-Lymphocytes (immunology)
  • Transaldolase (genetics, immunology)

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