31P nuclear magnetic resonance study of phospholipid metabolites in ischemic liver.

Abstract

To assess the metabolic alterations induced by normothermic hepatic ischemia, 31P nuclear magnetic resonance analysis was performed on liver samples using perchloric acid extraction. In particular, phosphomonoesters and phosphodiesters, the intermediary metabolites of membrane phospholipid turnover, were characterized precisely and quantitated. Phosphocholine and phosphoethanolamine, the precursors of phospholipid anabolism, did not change, while the phosphodiesters decreased. In contrast, alpha-glycerophosphate, which is both a precursor of phospholipid synthesis and the intermediary product of phospholipid degradation, markedly increased following 30 min of normothermic ischemia. These findings suggest that cellular phospholipids are actively degraded during normothermic hepatic ischemia.

• Authors: T Hachisuka, S Nakayama, T Tomita, H Takagi
• Journal: The Journal of surgical research (J Surg Res) Vol. 53 Issue 3 Pg. 251-6 (Sep 1992) ISSN: 0022-4804 [Print] United States
• PMID: 1528050 (Publication Type: Journal Article)

Chemical References

• Glycerophosphates
• Nucleotides
• Phosphates
• Phosphatidylcholines
• Phosphatidylethanolamines
• Phospholipids
• Adenosine Triphosphate
• alpha-glycerophosphoric acid

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Glycerophosphates (metabolism)
• Ischemia (metabolism)
• Liver (blood supply, metabolism)
• Magnetic Resonance Spectroscopy
• Male
• Mice
• Nucleotides (metabolism)
• Phosphates (metabolism)
• Phosphatidylcholines (metabolism)
• Phosphatidylethanolamines (metabolism)
• Phospholipids (metabolism)