Abstract |
Stromal cells play an important role in regulating epithelial malignancies through diffusible factors and adhesion. Modulation of the tumor-stromal cell interaction is an attractive target for new antitumor strategies. To screen for a modulator of the interaction, we have now developed a quantitative colorimetric assay for measurement of tumor cell growth in coculture with stromal cells using rhodanile blue dye. Rhodanile blue specifically stained cytokeratin-positive tumor cells in the coculture. When human prostate carcinoma cells LNCaP, PC-3 and DU-145 were cocultured with normal prostate stromal cells (PrSC) in a microplate, growth of the prostate cancer cells in the coculture was selectively measured by the rhodanile blue staining method. Using this system, we searched for a modulator of the tumor-stromal cell interaction among clinically used drugs and natural products. As a result, we found that 5-fluorouracil, bleomycin and phthoxazolin A inhibit prostate cancer cell growth more strongly in coculture with PrSC than that in monoculture. Without need to pre-label cells and transfect a marker gene, our new method is simple, rapid and thus useful for screening for modulators of the tumor-stromal cell interaction. Furthermore, our results suggest that low molecular weight compounds modulate the tumor-stromal cell interaction.
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Authors | Manabu Kawada, Yuya Yoshimoto, Kazuhisa Minamiguchi, Hiroyuki Kumagai, Tetsuya Someno, Tohru Masuda, Masaaki Ishizuka, Daishiro Ikeda |
Journal | Anticancer research
(Anticancer Res)
2004 May-Jun
Vol. 24
Issue 3a
Pg. 1561-8
ISSN: 0250-7005 [Print] Greece |
PMID | 15274323
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fatty Alcohols
- Oxazoles
- Polyunsaturated Alkamides
- Xanthenes
- Bleomycin
- phthoxazolin
- rhodanile blue
- Fluorouracil
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Topics |
- Bleomycin
(pharmacology)
- Cell Communication
(drug effects, physiology)
- Cell Division
(drug effects, physiology)
- Cell Line, Tumor
- Coculture Techniques
- Colorimetry
- Epithelial Cells
(drug effects, pathology)
- Fatty Alcohols
(pharmacology)
- Fluorouracil
(pharmacology)
- Humans
- Male
- Oxazoles
(pharmacology)
- Polyunsaturated Alkamides
- Prostate
(cytology, drug effects)
- Prostatic Neoplasms
(drug therapy, pathology)
- Stromal Cells
(cytology, drug effects)
- Xanthenes
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