Disease-specific B Cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens.
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| Abstract |
Severe acute respiratory syndrome (SARS) has emerged as a highly contagious, sometimes fatal disease. To find disease-specific B cell epitopes, phage-displayed random peptide libraries were panned on serum immunoglobulin (Ig) G antibodies from patients with SARS. Forty-nine immunopositive phage clones that bound specifically to serum from patients with SARS were selected. These phageborne peptides had 4 consensus motifs, of which 2 corresponded to amino acid sequences reported for SARS-associated coronavirus (SARS-CoV). Synthetic peptide binding and competitive-inhibition assays further confirmed that patients with SARS generated antibodies against SARS-CoV. Immunopositive phage clones and epitope-based peptide antigens demonstrated clinical diagnostic potential by reacting with serum from patients with SARS. Antibody-response kinetics were evaluated in 4 patients with SARS, and production of IgM, IgG, and IgA were documented as part of the immune response. In conclusion, B cell epitopes of SARS corresponded to novel coronavirus. Our epitope-based serologic test may be useful in laboratory detection of the virus and in further study of the pathogenesis of SARS.
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| Authors | I-Ju Liu, Po-Ren Hsueh, Chin-Tarng Lin, Chien-Yu Chiu, Chuan-Liang Kao, Mei-Ying Liao, Han-Chung Wu |
| Journal | The Journal of infectious diseases (J Infect Dis) Vol. 190 Issue 4 Pg. 797-809 (Aug 15 2004) ISSN: 0022-1899 [Print] United States |
| PMID | 15272409 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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