Abstract |
Hemolin is one of the haemolymph proteins most strongly induced upon bacterial infection in Lepidoptera. When we applied RNA interference (RNAi) to suppress Hemolin expression in the Chinese oak silk moth Antheraea pernyi, we discovered that Hemolin is induced by double-stranded RNA (dsRNA) per se. As dsRNA is recognized as a virus pattern molecule, we then investigated the effect of a baculovirus (ApNPV) infection. We found that Hemolin is induced and expressed with similar kinetics as upon dsRNA injection. Notably, no Attacin gene expression or antibacterial activity was recorded. When baculovirus and high amounts of dsRNA were coinjected, the viral symptoms appeared earlier with Hemolin dsRNA than with GFP dsRNA. This indicates that silencing of hemolin affected the progress of the viral infection.
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Authors | M Hirai, O Terenius, W Li, I Faye |
Journal | Insect molecular biology
(Insect Mol Biol)
Vol. 13
Issue 4
Pg. 399-405
(Aug 2004)
ISSN: 0962-1075 [Print] England |
PMID | 15271212
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- DNA, Complementary
- Immunoglobulins
- Insect Proteins
- Luminescent Proteins
- Proteins
- RNA, Double-Stranded
- attacin antibacterial protein, insect
- hemolin
- Green Fluorescent Proteins
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Topics |
- Animals
- Baculoviridae
- Base Sequence
- Blotting, Northern
- China
- DNA Primers
- DNA, Complementary
(genetics)
- Escherichia coli
- Female
- Gene Expression Regulation
- Green Fluorescent Proteins
- Hemolymph
(microbiology)
- Immunoglobulins
- Insect Proteins
(genetics, metabolism)
- Kinetics
- Larva
(metabolism, virology)
- Luminescent Proteins
(metabolism)
- Molecular Sequence Data
- Moths
(metabolism, microbiology, virology)
- Proteins
(metabolism)
- RNA, Double-Stranded
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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