Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification.

Abstract	Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2 approximately q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.
Authors	Yvonne T M Tsang, Yi-Mieng Chang, Xinyan Lu, Pulivarthi H Rao, Ching C Lau, Kwong-Kwok Wong
Journal	Cancer genetics and cytogenetics (Cancer Genet Cytogenet) Vol. 152 Issue 2 Pg. 124-8 (Jul 15 2004) ISSN: 0165-4608 [Print] United States
PMID	15262430 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	DNA, Neoplasm DNA-Binding Proteins Multienzyme Complexes PLAG1 protein, human lyn protein-tyrosine kinase src-Family Kinases Cysteine Endopeptidases Proteasome Endopeptidase Complex Adenosine Triphosphatases
Topics	Adenosine Triphosphatases (genetics) Chromosomes, Human, Pair 8 Cysteine Endopeptidases (genetics) DNA, Neoplasm (genetics) DNA-Binding Proteins (genetics) Gene Amplification Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Mixed Tumor, Malignant (genetics, pathology) Multienzyme Complexes (genetics) Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Proteasome Endopeptidase Complex Reverse Transcriptase Polymerase Chain Reaction Salivary Gland Neoplasms (genetics, pathology) src-Family Kinases (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!