Abstract |
Three series of cultured Wistar rat heart cells (10 treated and 10 controls x 3) were examined with a laser contraction-meter in a special chamber for anoxia to determine whether cytidine diphosphate choline ( CDPC), a membrane phospholipid precursor, can protect against total oxygen deprivation. Heart rate and force of contraction (inotropism) were monitored during a 40-minute period of hypoxia. CDPC in a concentration of 142 micrograms/ml-1 was added to the culture medium only during the anoxia period in series I, during the 3 days of culture in series II, and during the 3 days of culture and the 40-minute hypoxia period in series III. In series I, inotropism decreased by 21% versus 55% in control group (P less than 0.05). In series II, inotropism decreased by 25% versus 43% in control group (P less than 0.05). In series III, inotropism decreased by 22% versus 44% in control group (P less than 0.05). Compared with control cells, cells treated with CDPC during anoxia maintained a significantly greater inotropic state. The effect is greatest if the cells are weak, as in series I. CDPC may be a useful component of the cardioplegic mixture during cardiopulmonary bypass and in the treatment of myocardial ischemia.
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Authors | A Neidhardt, Y Costes, K Bachour, N Platonoff |
Journal | Clinical therapeutics
(Clin Ther)
1992 Jul-Aug
Vol. 14
Issue 4
Pg. 537-43
ISSN: 0149-2918 [Print] United States |
PMID | 1525788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media
- Cytidine Diphosphate Choline
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Topics |
- Animals
- Cell Hypoxia
(drug effects)
- Cells, Cultured
- Culture Media
- Cytidine Diphosphate Choline
(pharmacology)
- Myocardial Contraction
(drug effects)
- Myocardium
(cytology)
- Rats
- Rats, Inbred Strains
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