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Effect of cytidine diphosphate choline on anoxia tolerance of cultured myocardial cells.

Abstract
Three series of cultured Wistar rat heart cells (10 treated and 10 controls x 3) were examined with a laser contraction-meter in a special chamber for anoxia to determine whether cytidine diphosphate choline (CDPC), a membrane phospholipid precursor, can protect against total oxygen deprivation. Heart rate and force of contraction (inotropism) were monitored during a 40-minute period of hypoxia. CDPC in a concentration of 142 micrograms/ml-1 was added to the culture medium only during the anoxia period in series I, during the 3 days of culture in series II, and during the 3 days of culture and the 40-minute hypoxia period in series III. In series I, inotropism decreased by 21% versus 55% in control group (P less than 0.05). In series II, inotropism decreased by 25% versus 43% in control group (P less than 0.05). In series III, inotropism decreased by 22% versus 44% in control group (P less than 0.05). Compared with control cells, cells treated with CDPC during anoxia maintained a significantly greater inotropic state. The effect is greatest if the cells are weak, as in series I. CDPC may be a useful component of the cardioplegic mixture during cardiopulmonary bypass and in the treatment of myocardial ischemia.
AuthorsA Neidhardt, Y Costes, K Bachour, N Platonoff
JournalClinical therapeutics (Clin Ther) 1992 Jul-Aug Vol. 14 Issue 4 Pg. 537-43 ISSN: 0149-2918 [Print] United States
PMID1525788 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Culture Media
  • Cytidine Diphosphate Choline
Topics
  • Animals
  • Cell Hypoxia (drug effects)
  • Cells, Cultured
  • Culture Media
  • Cytidine Diphosphate Choline (pharmacology)
  • Myocardial Contraction (drug effects)
  • Myocardium (cytology)
  • Rats
  • Rats, Inbred Strains

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