Comparative studies of galactose-6-mustard and L-phenylalanine mustard on cell growth and cell cycle kinetics in vitro.

Abstract

The effect of galactose-6-mustard (G-6-M) on cell growth and cell cycle kinetics was studied in murine P388 leukemia and Chinese hamster ovary (CHO) cells in vitro and compared with the effect of L-phenylalanine mustard (L-PAM). The IC50 values of G-6-M for the P388 and CHO cells were 10 and 100 microM, respectively. No difference of the IC50 value of L-PAM (2 microM) between the two cell lines was found. The effect of G-6-M and L-PAM on cell kinetics was similar for the two cell lines at IC50 doses. The relative cell outflow from the G2 stage was inhibited to a higher extent than the relative cell outflow from the S phase. The relative cell outflow from the G1 stage was only partly inhibited. These results are discussed in relation to growth conditions, differences in DNA repair capacity, and cellular uptake of G-6-M between P388 and CHO cells.

• Authors: K Stenberg, S Skog, B Tribukait, A L Borg
• Journal: Anti-cancer drugs (Anticancer Drugs) Vol. 3 Issue 2 Pg. 125-31 (Apr 1992) ISSN: 0959-4973 [Print] England
• PMID: 1525389 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Alkylating Agents
• DNA, Neoplasm
• Tritium
• C6-galactose mustard
• Galactosamine
• Melphalan
• Thymidine

Topics

• Alkylating Agents (pharmacology)
• Animals
• CHO Cells (cytology, drug effects, metabolism)
• Cell Cycle (drug effects)
• Cell Division (drug effects)
• Cricetinae
• DNA, Neoplasm (metabolism)
• Fibroblasts (cytology, drug effects)
• Galactosamine (analogs & derivatives, pharmacology)
• Leukemia P388 (drug therapy, metabolism, pathology)
• Melphalan (pharmacology)
• Mice
• Thymidine (metabolism)
• Tritium
• Tumor Cells, Cultured (drug effects)