| Abstract | Mastitis is a major cause of economic loss to the dairy industry. Lactoferrin (Lf) is known to contribute to resistance against bacterial infections. Hence, we decided to characterize the relevance between mastitis resistance and the variants of Lf gene. By using PCR-SSCP, five fragments within 5' region and all exons of bovine lactoferrin gene were amplified and identified the nucleotide diversity. For the five segments within the 5'-region: Lf5'-1, Lf5'-2, Lf5'-3, Lf5'-4, and Lf5'-5 from upstream to downstream, we found that three had base variation. Totally, mutations were observed in Lf5'-1, Lf5'-3, and Lf5'-5, exons 4, 8, 9, 11, 15, and intron 4. We analyzed the effects of all mutated loci on milk production traits with least squares method. |
| Authors | Guo-Hua Li, Yuan Zhang, Dong-Xiao Sun, Ning Li
(Affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, PR China.)
|
| Journal | Animal biotechnology
(Anim Biotechnol)
Vol. 15
Issue 1
Pg. 67-76
(May 2004)
ISSN: 1049-5398 United States |
| PMID | 15248601
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
|
| Topics |
- Animals
- Base Sequence
- Cattle
- Cell Count
(veterinary)
- DNA
(chemistry, genetics)
- Female
- Lactoferrin
(genetics)
- Least-Squares Analysis
- Mastitis, Bovine
(genetics)
- Molecular Sequence Data
- Polymerase Chain Reaction
(veterinary)
- Polymorphism, Single Nucleotide
- Polymorphism, Single-Stranded Conformational
- Sequence Alignment
|
