Determination of tetramine in marine gastropods by liquid chromatography/electrospray ionization-mass spectrometry.
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| Abstract |
Tetramine (tetramethylammonium ion) is found at high levels (several mg/g) in the salivary gland of buccinid gastropods and has been involved in numerous poisoning incidents after ingestion of those gastropods. A sensitive and selective determination method for tetramine, which is based on a combination of liquid chromatography (LC) and electrospray ionization-single quadrupole mass spectrometry (ESI-MS), was developed. Following separation by LC on a cation-exchange column, tetramine was easily detected by simultaneous monitoring of a molecular ion (m/z 74) at a cone voltage of 30 V and a fragment ion (m/z 58) at 70 V. A linear calibration curve was obtained in the range of 0.1-100 ng by plotting the peak areas of the molecular ion versus the amounts of tetramine. Spiking experiments demonstrated that tetramine in gastropod tissues can be determined by the LC/ESI-MS method, without being affected by sample matrices as well as the extration procedure. Applications of the new method to gastropod samples revealed that a small amount of tetramine is contained even in mid-gut gland and muscle and that tetramine in the salivary gland diffuses to other tissues during boiling and slow thawing.
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| Authors | Yoko Kawashima, Yuji Nagashima, Kazuo Shiomi |
| Journal | Toxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 44 Issue 2 Pg. 185-91 (Aug 2004) ISSN: 0041-0101 [Print] England |
| PMID | 15246768 (Publication Type: Journal Article, Validation Study) |
| Chemical References |
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