Abstract | BACKGROUND: METHODS: 243 women undergoing IVF/ICSI treatment donated 353 oocytes in a multicentre, prospective, randomized, double blind, four-arm, controlled trial performed at Danish and Swedish public and private IVF centers. Metaphase II oocytes were randomly assigned to: FF-MAS 5 microM, FF-MAS 20 microM, ethanol 0.2% (vehicle control) or water for injection (inert control). The exposure regimen of FF-MAS to the human oocytes was 4 h prior to fertilization by ICSI and 20 h exposure post ICSI. The primary endpoint was the incidence of numerical chromosomal abnormalities. Secondary endpoints were cleavage rate and pre-embryo quality. RESULT: On the pre-embryo level, no significant differences in chromosomal abnormality rate were observed among the four groups. However, the percentage of uniformly normal pre-embryos was significantly lower in the pooled FF-MAS group (5 microM: 12% and 20 microM: 17%) than in the pooled control group (inert control 32% and vehicle control 42%). A high level of mosaicism (41-60%) was found in all groups. At the blastomere level, the percentage of blastomeres categorized as normal was significantly lower in the FF-MAS 5 microM group (41%) and the FF-MAS 20 microM (29%) group versus the inert (52%) and the vehicle (61%) groups. Significantly reduced cleavage and good quality pre-embryo rates were found in both FF-MAS groups. CONCLUSION:
FF-MAS increased the rate of aneuploidy and had detrimental effects on cleavage and pre-embryo development, when exposed both before and after fertilization.
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Authors | Christina Bergh, Anne Loft, Kersti Lundin, Sören Ziebe, Lars Nilsson, Matts Wikland, Christian Gröndahl, J-C Arce, CEMAS II Study Group |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 19
Issue 9
Pg. 2109-17
(Sep 2004)
ISSN: 0268-1161 [Print] England |
PMID | 15243006
(Publication Type: Clinical Trial, Clinical Trial, Phase I, Journal Article, Multicenter Study, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cholestenes
- 4,4-dimethylcholesta-8,14,24-trienol
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Topics |
- Adult
- Blastocyst
(cytology, physiology, ultrastructure)
- Blastomeres
(cytology, physiology)
- Cells, Cultured
- Cholestenes
(administration & dosage, analysis, pharmacology)
- Chromosome Aberrations
(statistics & numerical data)
- Cleavage Stage, Ovum
(drug effects)
- Cytogenetic Analysis
- Cytoplasm
(ultrastructure)
- Dose-Response Relationship, Drug
- Double-Blind Method
- Embryonic Development
- Female
- Fertilization
- Fertilization in Vitro
- Follicular Fluid
(chemistry)
- Humans
- Metaphase
- Mosaicism
- Oocytes
(drug effects)
- Osmolar Concentration
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