Abstract |
Here we report the cloning of the rat 90K, a homolog of the mouse cyclophilin C-associated protein/mouse adherent macrophage and human 90K. The protein is constitutively expressed by FRTL-5 thyrocytes, and its levels are modulated by TSH, insulin/ IGF-I, and gamma-interferon. Transfection of the cells with 90K cDNA or exposure to purified 90K resulted in a significant increase of the expression of major histocompatibility complex class I but not class II antigens. An increased expression of 90K was obtained after viral infection or introduction into the cells of fragments of viral, bacterial, or mammalian double-strand polynucleotides. The increase was sequence independent, not CpG mediated, and associated with the expression of molecules characterizing antigen-presenting-cell phenotype. The present data along with results from previous studies suggest that 90K plays an important role in the maintenance of an appropriate level of immune response.
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Authors | Antonino Grassadonia, Nicola Tinari, Bruno Fiorentino, Koichi Suzuki, Minoru Nakazato, Michele De Tursi, Cesidio Giuliani, Giorgio Napolitano, Dinah S Singer, Stefano Iacobelli, Leonard D Kohn |
Journal | Endocrinology
(Endocrinology)
Vol. 145
Issue 10
Pg. 4728-36
(Oct 2004)
ISSN: 0013-7227 [Print] United States |
PMID | 15231701
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carrier Proteins
- Extracellular Matrix Proteins
- Histocompatibility Antigens Class I
- Hormones
- Lgals3bp protein, rat
- Polynucleotides
- Proteins
- RNA, Messenger
- Interferon-gamma
- DNA
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Topics |
- Amino Acid Sequence
- Animals
- Carrier Proteins
- Cell Line
- Cloning, Molecular
- DNA
(pharmacology)
- Extracellular Matrix Proteins
- Herpes Simplex
(metabolism)
- Histocompatibility Antigens Class I
(metabolism)
- Hormones
(pharmacology)
- Humans
- Interferon-gamma
(pharmacology)
- Molecular Sequence Data
- Polynucleotides
(genetics, pharmacology)
- Proteins
(genetics, physiology)
- RNA, Messenger
(metabolism)
- Rats
- Sequence Homology, Amino Acid
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