Gene deletion of dopamine beta-hydroxylase and alpha1-adrenoceptors demonstrates involvement of catecholamines in vascular remodeling.

Abstract

In vitro studies have shown that stimulation of alpha1-adrenoceptors (ARs) directly induces proliferation, hypertrophy, and migration of arterial smooth muscle cells and adventitial fibroblasts. In vivo studies confirmed these findings and showed that catecholamine trophic activity becomes excessive after experimental balloon injury and contributes to neointimal growth, adventitial thickening, and lumen loss. However, past studies have been limited by selectivity of pharmacological agents. The aim of this study, in which mice devoid of norepinephrine and epinephrine synthesis [dopamine beta-hydroxylase (DBH-/-)] or deficient in alpha1-AR subtypes expressed in murine carotid (alpha1B-AR-/- and alpha1D-AR-/-) were used, was to test the hypothesis that catecholamines contribute to wall hypertrophy after injury. At 3 wk after injury of wild-type mice, lumen area and carotid circumference increased significantly, and hypertrophy of media and adventitia was in excess of that needed to restore circumferential wall stress to normal. In DBH-/- and alpha1B-AR-/- mice, increases in lumen area, circumference, and hypertrophy of the media and adventitia were reduced by 50-91%, resulting in restoration of wall tension to nearly normal (DBH-/-) or normal (alpha1B-AR-/-). In contrast, in alpha1D-AR-/- mice, increases in lumen area, circumference, and wall hypertrophy were unaffected and wall thickening remained in excess of that required to return tension to normal. When examined 5 days after injury, proliferation and leukocyte infiltration were inhibited in DBH-/- mice. These studies suggest that the trophic effects of catecholamines are mediated primarily by alpha1B-ARs in mouse carotid and contribute to hypertrophic growth after vascular injury.

• Authors: Hua Zhang, Susanna Cotecchia, Steven A Thomas, Akito Tanoue, Gozoh Tsujimoto, James E Faber
• Journal: American journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 287 Issue 5 Pg. H2106-14 (Nov 2004) ISSN: 0363-6135 [Print] United States
• PMID: 15231500 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Catecholamines
• Protein Isoforms
• Receptors, Adrenergic, alpha-1
• Dopamine beta-Hydroxylase

Topics

• Animals
• Apoptosis
• Carotid Arteries (metabolism, pathology, physiopathology)
• Carotid Artery Injuries (metabolism, pathology, physiopathology)
• Catecholamines (metabolism)
• Cell Division
• Dopamine beta-Hydroxylase (genetics)
• Female
• Gene Deletion
• Leukocytes (pathology)
• Male
• Mice
• Mice, Knockout
• Protein Isoforms (genetics, metabolism)
• Receptors, Adrenergic, alpha-1 (genetics, metabolism)