Abstract |
Whereas the complete genome of Coxiella burnetii (C.b.), the etiological agent of Q-fever, has recently been published (Seshadri et al., Proc. Natl. Acad. Sci. USA. 100, 5455-5460, 2003), the C.b. proteome is still under study. Using the bioinformatic approach, we found in total 309 proteins on two dimensional electroctrophoretic images of C.b. whole cell lysates. Eighteen major protein species were subjected to peptide mass fingerprinting and identified as the products of 6 known open reading frames (ORFs): the chaperone DnaK (heat shock 70 K protein), chaperonin 60 K (GroEL protein, heat shock protein B), DnaJ-like protein djlA (mucoidy activation protein mucZ), elongation factor Ts (EF-Ts), ribosomal protein L7/L12, and chaperonin 10 K (GroES protein, heat shock protein A).
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Authors | L' Skultéty, L Hernychová, R Toman, M Kroca, J Stulík, A Macela |
Journal | Acta virologica
(Acta Virol)
Vol. 48
Issue 1
Pg. 29-33
( 2004)
ISSN: 0001-723X [Print] Slovakia |
PMID | 15230472
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Broncho-Vaxom
- Cell Extracts
- Heat-Shock Proteins
- Proteome
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Topics |
- Bacteria
- Bacterial Proteins
(analysis)
- Cell Extracts
(analysis)
- Coxiella burnetii
(chemistry, genetics)
- Genome, Bacterial
- Heat-Shock Proteins
(metabolism)
- Peptide Mapping
(methods)
- Proteome
(analysis)
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