Expression profile of eukaryotic translation factors in human cancer tissues and cell lines.

Abstract

Several studies have demonstrated the overexpression of certain eukaryotic translation factors in human cancer cell lines and in malignant tissues. In this study, with human cancer cell lines derived from lungs, breast, prostate, and skin, we have examined the expression profile of 36 translation factors consisting of 27 initiation factors, 8 elongation factors, and 1 termination factor. Translation initiation factors 2C2 and 4E1 and translation elongation factors 1A2 and 1delta were found overexpressed (2- to 2000-fold) in many of the cancer cell lines compared to their corresponding normal cell lines. Among the translation factors analyzed, translation elongation factor 1A2 exhibited the most significant alteration in expression: 10- to 2000-fold overexpression was noticed in nine out of ten cancer cell lines analyzed. Whether the overexpression of translation elongation factor 1A2 can be used as a potential tumor marker was tested with the cancer profiling array (BD Biosciences, Palo Alto, CA) consisting of 241 paired cDNA samples generated from 13 different cancer/noncancer tissue types. Overexpression of translation elongation factor 1A2 was noticed in several tumor tissue samples, most notably in the human colon cancer samples which exhibited at least a twofold overexpression among 35% of the samples analyzed. Besides colon, tumor samples derived from lungs, kidney, rectum, and ovary also exhibited more than a twofold overexpression of translation elongation factor 1A2 in at least 20% of the samples analyzed. These results indicate that human carcinogenesis is often associated with alterations in the expression of various translation factors especially the overexpression of eukaryotic translation elongation factor 1A2.

• Authors: Pius Joseph, Christina M O'Kernick, Sreekumar Othumpangat, Yi-Xiong Lei, Bao-Zhu Yuan, Tong-Man Ong
• Journal: Molecular carcinogenesis (Mol Carcinog) Vol. 40 Issue 3 Pg. 171-9 (Jul 2004) ISSN: 0899-1987 [Print] United States
• PMID: 15224349 (Publication Type: Journal Article)
• Copyright: Copyright 2004 Wiley-Liss, Inc.

Chemical References

• Eukaryotic Initiation Factors

Topics

• Breast Neoplasms (genetics, metabolism)
• Eukaryotic Initiation Factors (metabolism)
• Female
• Gene Expression Profiling
• Humans
• Lung Neoplasms (genetics, metabolism)
• Male
• Prostatic Neoplasms (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Skin Neoplasms (genetics, metabolism)
• Tumor Cells, Cultured