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In vitro-deranged intestinal immune response to gliadin in type 1 diabetes.

Abstract
Dietary gluten has been associated with an increased risk of type 1 diabetes. We have evaluated inflammation and the mucosal immune response to gliadin in the jejunum of patients with type 1 diabetes. Small intestinal biopsies from 17 children with type 1 diabetes without serological markers of celiac disease and from 50 age-matched control subjects were examined by immunohistochemistry. In addition, biopsies from 12 type 1 diabetic patients and 8 control subjects were cultured with gliadin or ovalbumin peptic-tryptic digest and examined for epithelial infiltration and lamina propria T-cell activation. The density of intraepithelial CD3(+) and gammadelta(+) cells and of lamina propria CD25(+) mononuclear cells was higher in jejunal biopsies from type 1 diabetic patients versus control subjects. In the patients' biopsies cultured with peptic-tryptic gliadin, there was epithelial infiltration by CD3(+) cells, a significant increase in lamina propria CD25(+) and CD80(+) cells and enhanced expression of lamina propria CD54 and crypt HLA-DR. No such phenomena were observed in control subjects, even those with celiac disease-associated HLA haplotypes. In conclusion, signs of mucosal inflammation were present in jejunal biopsies from type 1 diabetic patients, and organ culture studies indicate a deranged mucosal immune response to gliadin.
AuthorsRenata Auricchio, Francesco Paparo, Maria Maglio, Adriana Franzese, Francesca Lombardi, Giuliana Valerio, Gerardo Nardone, Selvaggia Percopo, Luigi Greco, Riccardo Troncone
JournalDiabetes (Diabetes) Vol. 53 Issue 7 Pg. 1680-3 (Jul 2004) ISSN: 0012-1797 [Print] United States
PMID15220190 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • HLA Antigens
  • Gliadin
Topics
  • Adolescent
  • Antibody Formation
  • Biopsy
  • Case-Control Studies
  • Child
  • Diabetes Mellitus, Type 1 (immunology)
  • Female
  • Gliadin (immunology)
  • HLA Antigens (analysis, classification)
  • Humans
  • Immunohistochemistry
  • Intestinal Mucosa (immunology)
  • Jejunum (immunology, pathology)
  • Male
  • Organ Culture Techniques

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