A prospective controlled evaluation of TEST-yolk buffer in the preparation of sperm for human in vitro fertilization in suspected cases of male infertility.

Abstract

OBJECTIVE:

To evaluate sperm preincubation in tes and tris (TEST)-yolk buffer as a potential enhancing agent of fertilizing capacity of sperm during human in vitro fertilization (IVF).

DESIGN:

Oocytes obtained during IVF were divided into two groups: one group fertilized with TEST-yolk buffer-treated sperm and the other group with standard-prepared sperm.

SETTING:

The University of Southern California IVF Program.

PATIENTS:

Thirty-nine couples with suspected male factor infertility undergoing IVF.

INTERVENTIONS:

Preinsemination incubation of sperm in TEST-yolk buffer for 24 hours.

MAIN OUTCOME MEASURES:

Fertilization rates in vitro.

RESULTS:

TEST-yolk buffer-treated sperm fertilized 140 of 241 oocytes (58%), whereas control sperm fertilized 108 of 251 oocytes (43%). Of four couples who had previously failed to fertilize during IVF, one fertilized with both specimens and three fertilized with TEST-yolk buffer-treated sperm only.

CONCLUSIONS:

TEST-yolk buffer pretreatment of sperm for 24 hours results in higher fertilization rates during IVF among suspected male factor patients.

• Authors: R J Paulson, M V Sauer, M M Francis, T M Macaso, R A Lobo
• Journal: Fertility and sterility (Fertil Steril) Vol. 58 Issue 3 Pg. 551-5 (Sep 1992) ISSN: 0015-0282 [Print] United States
• PMID: 1521651 (Publication Type: Journal Article)

Chemical References

• Tromethamine

Topics

• Egg Yolk
• Embryo Transfer
• Female
• Fertilization in Vitro
• Humans
• Infertility, Male (therapy)
• Male
• Pregnancy
• Prospective Studies
• Spermatozoa (physiology)
• Tromethamine