Abstract | BACKGROUND: METHODS: The effect of the compounds on cell cycle was determined using FACS and western blot analysis of cell cycle proteins. Hollow fibre assay was used to determine if the compounds had the same effect on the cell cycle in vitro and in vivo. RESULTS: Our results from the in vitro and in vivo experiments show that both the compounds block the cell cycle at the G0-G1 phase through the induction of the cell cycle inhibitor, p27, and the concomitant decrease in the levels of Cdk4. CONCLUSION: The results show that the novel semi-synthetic analog, DRF3188, and andrographolide bring about the anti cancer activity by a similar mechanism.
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Authors | Chitkala Satyanarayana, Dhanavanthri S Deevi, R Rajagopalan, Nanduri Srinivas, Sriram Rajagopal |
Journal | BMC cancer
(BMC Cancer)
Vol. 4
Pg. 26
(Jun 18 2004)
ISSN: 1471-2407 [Electronic] England |
PMID | 15207007
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- DRF3188
- Diterpenes
- andrographolide
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Breast Neoplasms
(drug therapy, pathology)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Diterpenes
(pharmacology)
- Flow Cytometry
- Humans
- Mice
- Xenograft Model Antitumor Assays
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