Abstract |
DNA-based immunization has shown to be a viable alternative approach to induce protective immunity against Brucella abortus infection. However, the use of a unique gene may not be sufficient to induce full protection. Therefore, a new strategy based on library immunization has been described to improve the level of protection against different pathogens and to identify new protective genes. In the present study, a B. abortus library was subcloned into the mammalian expression vector pCMV-Ubi. This plasmid was designed to create a fusion between the gene of interest with ubiquitin. The analysis of this Brucella-library showed approximately 72% of clones containing inserts with an average size of 500-2000 bp. Further, homology searches were performed using the BLASTn program, and all sequenced clones showed homology with Brucella genes, as expected. BALB/c mice immunised intramuscularly with the Brucella genomic expression library showed a strong specific total IgG antibody response to a Brucella protein extract, with production of IgG1 and IgG2a isotypes. Regarding cellular immunity, high levels of IFN-gamma and no IL-4 were detected in primed mouse splenocytes and partial protection against infection was reached in animals vaccinated with the Brucella library compared to the control group.
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Authors | Sophie Y Leclercq, Sergio Costa Oliveira |
Journal | Journal of drug targeting
(J Drug Target)
Vol. 11
Issue 8-10
Pg. 531-8
( 2003)
ISSN: 1061-186X [Print] England |
PMID | 15203922
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin G
- Tumor Necrosis Factor-alpha
- Vaccines, DNA
- Interleukin-4
- Interferon-gamma
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Topics |
- Animals
- Antibody Formation
- Brucella abortus
(genetics, immunology)
- Brucellosis
(immunology, prevention & control)
- Female
- Gene Library
- Genome, Bacterial
- Immunity, Cellular
- Immunoglobulin G
(blood)
- Interferon-gamma
- Interleukin-4
(biosynthesis)
- Mice
- Mice, Inbred BALB C
- Tumor Necrosis Factor-alpha
(biosynthesis)
- Vaccines, DNA
(administration & dosage, immunology)
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