Abstract | BACKGROUND: METHODS: Male Sprague-Dawley rats were rendered nephrotic by two sequential injections of puromycin aminonucleoside (130 mg/kg on day 1 and 60 mg/kg on day 14) and studied on day 30. Placebo-treated rats served as controls. Hepatic DGAT-1 and DGAT-2 mRNA abundance and enzymatic activity were measured. RESULTS: The nephrotic group exhibited heavy proteinuria, hypoalbuminemia, hypercholesterolemia, hypertriglyceridemia, and marked elevation of VLDL concentration. Hepatic DGAT-1 mRNA, DGAT-1, and total DGAT activity were significantly increased, whereas DGAT-2 mRNA abundance and activity were unchanged in the nephrotic rats compared to the control animals. The functional significance of elevation of DGAT activity was illustrated by the reduction in microsomal free fatty acid concentration in the liver of nephrotic animals. CONCLUSION:
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Authors | Nosratola D Vaziri, Choong H Kim, Dennis Phan, Sara Kim, Kaihui Liang |
Journal | Kidney international
(Kidney Int)
Vol. 66
Issue 1
Pg. 262-7
(Jul 2004)
ISSN: 0085-2538 [Print] United States |
PMID | 15200432
(Publication Type: Journal Article)
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Chemical References |
- Lipids
- RNA, Messenger
- Acyltransferases
- Dgat1 protein, rat
- Diacylglycerol O-Acyltransferase
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Topics |
- Acyltransferases
(genetics, metabolism)
- Animals
- Diacylglycerol O-Acyltransferase
- Lipids
(blood)
- Liver
(enzymology)
- Male
- Nephrotic Syndrome
(blood, metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Up-Regulation
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