Polymorphism in the N-acetyltransferase 1 alleles NAT1*10 and NAT1*14A and cytological gradings of exfoliated urothelial cells in benzidine-exposed Chinese workers: discussion of ethnic differences.

Abstract

N-acetyltransferase 1 (NAT1) modifies bladder cancer risk in European populations exposed to aromatic amines in cigarette smoke. The present study was performed to investigate a possible association between NAT1*10 and NAT1*14A genotypes and bladder cancer risk in benzidine-exposed Chinese workers. Based on the cytological gradings of exfoliated urothelial cells according to Papanicolaou, an exposed research cohort was stratified into subgroups. An allele-specific PCR-based procedure was used to detect the polymorphism in the polyadenylation signal at the locus NAT1 T(1088)A. A nested PCR-RFLP procedure was conducted to differentiate NAT1*14A (T(1088)A, C(1095)A, and G(560)A) from NAT1*10 (T(1088)A, C(1095)A). No significantly different frequencies of homozygous and heterozygous NAT1*10 alleles were found among the subgroups with (i) gradings according to Papanicolaou < or = II (18.3 and 40.2%, respectively), (ii) higher gradings according to Papanicolaou (> II; 28.0 and 34.1%, respectively), and (iii) with bladder cancer (26.3 and 34.2%, respectively). The present data show that NAT1*10 neither displayed an association with an elevated grading of urothelial cells nor a clear impact on the risk for bladder cancer in benzidine-exposed Chinese workers. Discrepancies with the findings in European populations could point to ethnic differences in the disposition of aromatic amines.

• Authors: Wei Chao Guo, Guo Fang Lin, Ji Gang Chen, Klaus Golka, Jian Hua Shen
• Journal: Archives of toxicology (Arch Toxicol) Vol. 78 Issue 8 Pg. 425-9 (Aug 2004) ISSN: 0340-5761 [Print] Germany
• PMID: 15197537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzidines
• Carcinogens
• DNA, Neoplasm
• Isoenzymes
• Arylamine N-Acetyltransferase
• N-acetyltransferase 1

Topics

• Adult
• Arylamine N-Acetyltransferase (genetics)
• Benzidines (adverse effects)
• Carcinogens (adverse effects)
• Carcinoma, Transitional Cell (chemically induced, epidemiology, genetics)
• China (epidemiology)
• DNA, Neoplasm (biosynthesis, genetics)
• Ethnicity
• Female
• Gene Frequency
• Genotype
• Humans
• Isoenzymes (genetics)
• Male
• Occupational Exposure
• Papanicolaou Test
• Polymorphism, Genetic (genetics)
• Smoking (pathology)
• Urinary Bladder Neoplasms (chemically induced, epidemiology, genetics)
• Urothelium (cytology)
• Vaginal Smears