Abstract | AIM: METHODS: The expressed mutant SEB-K172E in inclusion body was denatured and renatured, and then isolated and purified. The anti- tumor activity of the mutant SEB-K172E was compared with wild-type SEB. RESULTS: Renaturation and purification method of the mutant SEB-K172E was developed. The anti- tumor activity of acquired mutant protein was ten times higher than that of wild-type SEB. CONCLUSION: The anti- tumor activity of the mutant SEB-K172E is much higher than that of wild-type SEB, suggesting that the mutant SEB-K172E may become a potential anti- tumor drug.
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Authors | Xiao-hong Deng, Yu-ling Zheng, Feng Yu, Bao-an Ning, Yong-qiang Jiang |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 20
Issue 3
Pg. 360-2
(May 2004)
ISSN: 1007-8738 [Print] China |
PMID | 15193239
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Enterotoxins
- Superantigens
- enterotoxin B, staphylococcal
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Topics |
- Antineoplastic Agents
(pharmacology)
- Enterotoxins
(biosynthesis, genetics, pharmacology)
- HT29 Cells
(drug effects)
- Humans
- Point Mutation
- Protein Renaturation
- Staphylococcus aureus
(genetics)
- Superantigens
(biosynthesis, genetics, pharmacology)
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