Abstract | OBJECTIVE: METHODS: One hundred and twenty male Sprague-Dawley rats were used in this study. The rats were divided into three groups: control, model and ZD-0892 groups. In the model and ZD-0892 groups, the rats were subjected to a single subcutaneous injection of monocrotaline (60 mg/kg) in the hind flank, while the rats in control group received an equivalent volume of 0.9% saline. From day 21, the rats in the ZD-0892 and model groups received twice-daily gavage tube feedings of either ZD-0892 at a dose of 240 mg/kg per day or its administration vehicle, while the rats in control group were subjected to an equivalent volume of 0.9% saline. On days 21, 28 and 35 post-injection, the elastolytic activity was measured with a fluorescence microplate reader and pulmonary artery pressure was detected via catheterization. Meanwhile, the lungs were evaluated morphologically, using the barium- gelatin perfusion technique. RESULTS: The injection of monocrotaline led to severe pulmonary hypertension in rats 21 days later and pulmonary artery elastolytic activity increased remarkably. A 1-week treatment with ZD-0892 resulted in declines in elastase activity. This was associated with significant declines in pulmonary artery pressure, decreases in muscularization of peripheral arteries and reductions in medial hypertrophy. After 2 weeks, elastase activity returned to normal level. Pulmonary artery pressure and structure were normalized. CONCLUSION:
|
Authors | Li-jun Fu, Ai-qing Zhou, Jie Shen, Wu Zhao, Fen Li |
Journal | Zhonghua er ke za zhi = Chinese journal of pediatrics
(Zhonghua Er Ke Za Zhi)
Vol. 42
Issue 5
Pg. 375-8
(May 2004)
ISSN: 0578-1310 [Print] China |
PMID | 15189699
(Publication Type: Comparative Study, English Abstract, Journal Article)
|
Chemical References |
- Pyrroles
- Sulfonamides
- ZD 0892
- Monocrotaline
- Pancreatic Elastase
|
Topics |
- Animals
- Hypertension, Pulmonary
(chemically induced, drug therapy)
- Male
- Monocrotaline
(toxicity)
- Pancreatic Elastase
(antagonists & inhibitors)
- Pulmonary Artery
(drug effects, pathology, physiopathology)
- Pyrroles
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Sulfonamides
(pharmacology)
|