Time-dependent morphological alterations of cold-stored small bowel in Euro-Collins and Ringer's lactate solutions.

Abstract

Small bowel is one of the organs that can in principle be transplanted. Optimum preservation of the organ is essential for the success of transplantation. The aim of the present study is the investigation of time-related morphological changes of rat small bowel during preservation in hypothermic Euro-Collins (EC) and Ringer's lactate (RL) solution using light microscopy and transmission electron microscopy to evaluate the integrity of intercellular complexes of mucosal epithelium, one of the tissues of the intestine that is most susceptible to ischemia. Small bowels were perfused with either EC, RL solution or physiological saline solution and were placed in the different preservation solutions at 4 degrees C for 0, 3, 6 and 12 h. The results of our study suggest that both preservation solutions are suitable for short-term preservation of the small bowel although RL solution is more effective than EC solution. However, we conclude that further improvement of preservation solutions and/or techniques are needed to perform long-term preservation.

• Authors: Susanne Oner, Feriha Ercan, Serap Arbak
• Journal: Acta histochemica (Acta Histochem) Vol. 106 Issue 3 Pg. 207-17 ( 2004) ISSN: 0065-1281 [Print] Germany
• PMID: 15186928 (Publication Type: Journal Article)

Chemical References

• Euro-Collins' solution
• Hypertonic Solutions
• Isotonic Solutions
• Ringer's Lactate

Topics

• Animals
• Cold Temperature
• Female
• Hypertonic Solutions (pharmacology)
• Intestinal Mucosa (drug effects, pathology, ultrastructure)
• Intestine, Small (drug effects, pathology, ultrastructure)
• Isotonic Solutions (pharmacology)
• Male
• Microscopy, Electron
• Models, Animal
• Organ Preservation (methods)
• Rats
• Rats, Wistar
• Ringer's Lactate
• Time Factors